Effects of dietary supplementation with an expressed fusion peptide bovine lactoferricin-lactoferrampin on performance, immune function and intestinal mucosal morphology in piglets weaned at age 21 d
| First Author: | Tang, Zhiru |
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| Abstract: | Lactoferrin has antimicrobial activity associated with peptide fragments lactoferricin (LFC) and lactoferrampin (LFA) released on digestion. These two fragments have been expressed in Photorhabdus luminescens as a fusion peptide linked to protein cipB. The construct cipB-LFC-LFA was tested as an alternative to antimicrobial growth promoters in pig production. Sixty piglets with an average live body weight of 5-42 (SEM 0.59) kg were challenged with enterotoxigenic Escherichia coli and randomly assigned to four treatment groups fed a maize-soyabean meal diet containing either no addition (C), cipB at 100 mg/kg (C + B), cipB-LFC-LFA at 100 mg/k (C + L) or colistin sulfate at 100mg/kg (C + CS) for 3 weeks. Compared with C, dietary supplementation with C + L for 3 weeks increased daily weight gain by 21%, increased recovery from diarrhoea, enhanced serum glutathione peroxidase (GPx), peroxidase (POD) and total antioxidant content (T-AOC), liver GPx, POD, superoxide dismutase and T-AOC, Fe, total Fe-binding capacity, IgA, IgG and IgM levels (P<0.05), decreased the concentration of E. coli in the ileum, caecum and colon (P<0.05), increased the concentration of lactobacilli and bifidobacteria in the ileum, caecum and colon (P< 0.05), and promoted development of the villus-crypt architecture of the small intestine. Growth performance was similar between C + L- and C + CS-supplemented pigs. The present results indicate that LFC-LFA is an effective alternative to the feed antibiotic CS for enhancing growth performance in piglets weaned at age 21 d. |
| Contact the author: | Yin, Yulong |
| Page Number: | 998-1005 |
| Issue: | 7 |
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| PubYear: | 2009 |
| Volume: | 101 |
| Unit code: | 161343 |
| Publication Name: | BRITISH JOURNAL OF NUTRITION |
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