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  Principal Investigators
Name:
ZHAO, Jian-Guo
Subject:
Tel/Fax:
+86-10-64806259  / 
E-mail:
zhaojg@ioz.ac.cn, zhaojervis@hotmail.com
Address:
The State Key Laboratory of Stem cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, 1 Beichen West Road, Chaoyang District, Beijing 100101, P.R.China
More:
Group of Reproduction and Development in Large Animals      
Resume:

Dr. Zhao received his Ph.D. in Animal genetics and breeding (2003) from Northeast Agricultural University and attended Institute of Medical Genetics at Shanghai Jiaotong University as an assistant researcher from 2003 to 2005. He had postdoctoral training in University of New Orleans of US with Dr. Barry Bavister (2005-2007). After that he worked in University of Missouri-Columbia/ National Swine Research and Resource Center as a Research Assistant Professor from 2007 to 2010. He joined the State Key Laboratory of Reproductive Biology of the Institute of Zoology, CAS, as a principle investigator, supported by CAS “100 Talents” Program in 2010.

Research Interests:

Dr. Zhao’s research focus on making genetic modifications in pigs by Somatic Cell Nuclear Transfer (SCNT) combined with transgenic/gene targeting as well as mutagenesis. Pigs are playing important role not only in agricultural production but also in biomedical research. Therefore the long term goal of making genetic modification in pigs is of improving the production efficiency or pork quality and safety as well as animal models for human disease and health. His lab conducts research in two main areas.
1. Reprogramming mechanism in SCNT and epigenetic regulations in the development of early stage embryos
2. New strategies of making genetic modifications in pigs

Awards and Honors:

Professional Activities:

Research Grants:

Selected Publications:
  1. Zheng Q§, Lin J§, Huang J§, Zhang H, Zhang R, Zhang X, Cao C, Hambly C, Qin G, Yao J, Song R, Jia Q, Wang X, Li Y, Zhang N, Piao Z, Ye R, Speakman JR, Wang H, Zhou Q, Wang Y*, Jin W*, Zhao J*. Proc Natl Acad Sci U S A. 2017, doi: 10.1073/pnas.1707853114.
  2. Zhang Y§, Xue Y§, Cao C§, Huang J, Hong Q, Hai T, Jia Q, Wang X, Qin G, Yao J, Wang X, Zheng Q, Zhang R, Li Y, Luo A, Zhang N, Shi G, Wang Y, Ying H, Liu Z, Wang H, Meng A, Zhou Q, Wei H*, Liu F* , Zhao J* . Thyroid hormone regulates hematopoiesis via the TR-KLF9 axis. Blood, 2017,blood-2017-05-783043.
  3. Huang J§, Wang Y*, Zhao J*. CRISPR editing in biological and biomedical investigation. J Cell Physiol. 2017.
  4. Hai T§, Cao C§, Shang H§, Guo W§, Mu Y§, Yang S, Zhang Y, Zheng Q, Zhang T, Wang X, Liu Y, Kong Q, Li K, Wang D, Qi M, Hong Q, Zhang R, Wang X, Jia Q, Wang X, Qin G, Li Y, Luo A, Jin W, Yao J, Huang J, Zhang H, Li M, Xie X, Zheng X, Guo K, Wang Q, Zhang S, Li L, Xie F, Zhang Y, Weng X, Yin Z, Hu K, Cong Y, Zheng P, Zou H, Xin L, Xia J, Ruan J, Li H, Zhao W, Yuan J, Liu Z, Gu W, Li M, Wang Y, Wang H* , Yang S*, Liu Z*, Wei H*, Zhao J*, Zhou Q*, Meng A*. Pilot study of large-scale production of mutant pigs by ENU mutagenesis. Elife, 2017, 6:e26248.
  5. Wang L§, Cao C§, Wang F, Zhao J*, Li W*. H2B ubiquitination: Conserved molecular mechanism, diverse physiologic functions of the E3 ligase during meiosis. Nucleus, 2017:1.
  6. Lin J§, Cao C§, Tao C§, Ye R, Dong M, Zheng Q, Wang C, Jiang X, Qin G, Yan C, Li K, Speakman JR, Wang Y*, Jin W*, Zhao J* . Cold adaptation in pigs depends on UCP3 in beige adipocytes. Journal of Molecular Cell Biology, 2017: 1-12.
  7. Jia Q§, Cao C§, Tang H, Zhang Y, Zheng Q, Wang X, Zhang R, Wang X, Luo A, Wei H, Meng A, Zhou Q, Wang H*, Zhao J* . A 2-bp insertion (c. 67_68insCC) in MC1R causes recessive white coat color in Bama miniature pigs. Journal of Genetics and Genomics, 2017, 44(4): 215-217.
  8. Song C§, Yao J§, Cao C, Liang X, Huang J, Han Z, Zhang Y, Qin G, Tao C, Li C, Yang H, Zhao J, Li K, Wang Y. PPARγ is regulated by miR-27b-3p negatively and plays an important role in porcine oocyte maturation. Biochemical and Biophysical Research Communications, 2016, 479(2): 224-230.
  9. Yao J, Huang J, Zhao J*. Genome editing revolutionize the creation of genetically modified pigs for modeling human diseases. Human Genetics, 2016, 135(9): 1093-1105.
  10.  Wang X§, Cao C§, Huang J§, Yao J, Hai T, Zheng Q, Wang X, Zhang H, Qin G, Cheng J, Wang Y, Yuan Z, Zhou Q, Wang H*, Zhao J*. One-step generation of triple gene targeted pigs using CRISPR/Cas9 system. Sci Rep. 2016 Feb 9;6:20620.
  11. Huang J§, Zhang H, Yao J, Qin G, Wang F, Wang X, Luo A, Zheng Q, Cao C, Zhao J*. BIX-01294 increases pig cloning efficiency by improving epigenetic reprogramming of somatic cell nuclei. Reproduction. 2016 Jan;151(1):39-49.
  12. Zhao J§. , Xu WJ, Ross JW., Walters EM., Butler SP., Whyte JJ., Kelso L., Fatemi M, Vanderslice NC., Giroux K., Spate LD., Samuel MS., Murphy CN., Wells KD., Masiello NC., Prather RS*. & Velander WH*. Engineering protein processing of the mammary gland to produce abundant hemophilia B therapy in milk . Sci Rep. 2015 Sep 21;5:14176.
  13. Huang J§, Zhang H, Wang X, Dobbs KB, Yao J, Qin G, Whitworth K, Walters EM, Prather RS*, Zhao J*. Impairment of Preimplantation Porcine Embryo Development by Histone Demethylase KDM5B Knockdown Through Disturbance of Bivalent H3K4me3-H3K27me3 Modifications. Biol Reprod. 2015 Mar; 92(3):72.
  14. Yao J§, Huang JJ, HaiTang , Wang XL, Qin GS, Zhang HY, Wu R, Xi J. J, Yuan Z, Zhao J*. Efficient bi-allelic gene knockout and site-specific knock-in mediated by TALENs in pigs. Sci Rep. 2014 Nov 5;4:6926.
  15. Whyte JJ*, Zhao JG*, Samuel M, Laughlin HM, Prather RS, Gene Targeting with Zinc Finger Nucleases to Produce Cloned eGFP Knockout Pigs. Mol Reprod Dev. 2011, Accepted.
  16. Zhao JG, Whyte JJ, Prather RS. The effect of epigenetic regulation on somatic cell nuclear transfer. Cell. Cell Tissue Res. 2010, 341(1):13-21.
  17. Zhao JG, Hao Y, Ross JW, Spate LD, Walters EM, Samuel MS, Rieke A, Murphy CN, Prather RS. Histone Deacetylase Inhibitors Improve In Vitro and In Vivo Developmental Competence of Somatic cell Nuclear Transfer Porcine Embryos. Cloning and stem cells, 2010;12(1):75-83
  18. Ross JW, Prather RS, Whyte JJ, Zhao JG. Cloning and transgenics: progress and new approaches. Invited review by Centre for Agricultural Bioscience International 2009,4, No. 38
  19. Zhao J, Ross JW, Hao Y, Spate LD, Walters EM, Samuel MS, Rieke A, Murphy CN, Prather RS. Significant Improvement in Cloning Efficiency of an Inbred Miniature Pig by Histone Deacetylase Inhibitor Treatment after Somatic Cell Nuclear Transfer. Biol Reprod. 2009 81: 525-530
  20. Zhao M, Isom SC, Lin H, Hao Y, Zhang Y, Zhao J, Whyte JJ, Dobbs KB, Prather RS. Tracing the stemness of porcine skin-derived progenitors (pSKP) back to specific marker gene expression. Cloning Stem Cells. 2009 ;11(1):111-22
  21. Miao YL, Sun QY, Zhang X, Zhao JG, Zhao MT, Spate L, Prather RS, Schatten H. Centrosome abnormalities during porcine oocyte aging. Environ Mol Mutagen. 2009; 50(8): 666-71
  22. Yang XY, Zhao JG, Li H, Liu HF, Huang Y, Huang SZ, Zeng F, Zeng YT. Effect of individual heifer oocyte donors on cloned embryo development in vitro. Anim Reprod Sci. 2008 104(1):28-37
  23. Yang XY, Li H, Ma QW, Yan JB, Zhao JG, Li HW, Shen HQ, Liu HF, Huang Y, Huang SZ, Zeng YT, Zeng F. Improved efficiency of bovine cloning by autologous somatic cell nuclear transfer. Reproduction. 2006 132(5):733-9
  24. Zhao JG, Li H, Kong X, Tang ZQ. Association analysis of SNPs in the chicken UCP gene with growth and body composition traits. Canadian Journal of Animal Science. 2006, 86:345-350
  25. Zhao JG, Li H, Wang YX, Meng H. The expression characterization of chicken uncoupling protein gene. Asian-Australasian Journal of Animal Science, 2005, 18 (11): 1552-1556
  26. Yang XY, Zhao JG, Li HW, Li H, Liu HF, Huang SZ, Zeng YT. Improve the in vitro development of cloned bovine embryos by use of holstein-chinese yellow hybrid recipient oocytes recovered by ovum pick up. Theriogenology. 2005, 64(6):1263-72
  27. Meng H, Li H, Zhao JG and Gu ZL. Differential expression of peroxisome proliferator-activated receptors alpha and gamma gene in various chicken tissues. Domestic Animal Endocrinology, 2005, 28:105-110