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Title: Rat Embryonic Stem Cells Produce Fertile Offspring Through Tetraploid Complementation
Author: Tian-Da Lia, Gui-Hai Fenga, Yu-Fei Lia, Mei Wang, Jun-Jie Mao, Jia-Qiang Wang, Xin Li, Xue-Peng Wang, Bin Qu, Le-Yun Wang, Xin-Xin Zhang, Hai-Feng Wan, Tong-Tong Cui, Cong Wan, Lei Liu, Xiao-Yang Zhao, Bao-Yang Hu, Wei Li, Qi Zhou
Abstract: Pluripotency of embryonic stem cells (ESCs) can be functionally assessed according to the developmental potency. Tetraploid complementation, through which an entire organism is produced from the pluripotent donor cells, is taken as the most stringent test for pluripotency. It remains unclear whether ESCs of other species besides mice can pass this test. Here we show that the rat ESCs derived under 2i (two small molecule inhibitors) conditions at very early passages are able to produce fertile offspring by tetraploid complementation. However, they lose this capacity rapidly during culture due to a nearly complete loss of genomic imprinting. Our findings support that the naïve ground state pluripotency can be captured in rat ESCs but also point to the species-specific differences in its regulation and maintenance, which have implications for the derivation and application of naïve pluripotent stem cells in other species including human.
Corresponding author: Qi Zhou
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PubYear: 2017
Issue: DOI: 10.1073/pnas.1708710114
Journal: Proceedings of the National Academy of Sciences
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URL: http://www.pnas.org/content/early/2017/10/20/1708710114.full