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Title: Retinoic Acid Is Sufficient for the In Vitro Induction of Mouse Spermatocytes
Author: Si Wang, Xiuxia Wang, Longfei Ma, Xiwen Lin, Daoqin Zhang, Zhen Li, Yujian Wu, Chunwei Zheng, Xue Feng, Shangying Liao, Yanmin Feng, Jian Chen, Xiangjing Hu, Min Wang, Chunsheng Han
Abstract: Meiosis is the key step in gametogenesis. However, the mechanism of mammalian meiosis remains poorly understood due to the lack of an in vitro model. Here, we report that retinoic acid (RA) is sufficient for inducing leptotene/zygotene spermatocytes from cultured mouse spermatogonial stem cells. Multiple genes regulated by RA were identified by RNA sequencing. RA in combination with pup Sertoli cell co-culture resulted in a higher induction efficiency of 28%. Comparisons in the transcriptomic profiles of the induced spermatogenic cells and the isolated ones revealed the progressive induction of the germ cells. Using this model, we showed that Stra8, Agpat3, Fam57a, Wdr91, and Sox30 contributed to the proliferation and meiosis initiation differentially. In conclusion, we have efficiently generated spermatocytes using an RA/pup Sertoli cell-based in vitro model and provided proof-of-concept evidence for its application in identifying genes involved in mammalian meiosis.
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PubYear: 2016
Issue: DOI: 10.1016/j.stemcr.2016.05.013
Journal: Stem Cell Reports
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URL: http://www.cell.com/stem-cell-reports/abstract/S2213-6711(16)30068-6