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汪迎春


汪迎春 博士, 研究员, 博士生导师
  
       1994年获兰州大学生物系生态学及环境生物学学士;1997年获中科院遗传研究所遗传学硕士;2003年获Iowa State University遗传学,生物信息及计算生物学双专业博士学位(Co-major. 20032006年,为The Scripps Research Institute 免疫系博士后;20062008年,为University of California, San Diego病理系及癌症研究中心博士后。2008-2009年,为University of California, San Diego病理系Assistant Project Scientist. 200911月加入中国科学院遗传与发育生物学研究所,入选中国科学院"百人计划"



研究方向

 

1.运动细胞的蛋白质组学

        细胞在外界化学因子的诱导下会产生形态变化和定向运动(Chemotaxis or Directional cell migration). 这个反应对生物生长发育过程中的一些重要的生理及病理过程起着至关重要的作用.如癌细胞的转移与扩散就是一种定向性细胞运动.我们将应用功能蛋白质组学研究运动细胞(包括癌细胞和神经细胞)在运动过程中的形态控制和信号转导机制. 并找出控制细胞运动的关键性蛋白和关键性翻译后修饰, 为与细胞运动相关的疾病的诊断和治疗提供靶标。

 

2. 生物信息学及计算生物学

        我们的生物信息及计算生物学研究主要是用来服务白质组学研究。我们将开发一些应用软件来处理大规模的蛋白质组学数据和进行功能性预测. 同时我们建立一个关于蛋白质磷酸化位点的网络数据库 (a web-based relational database). 这个数据库将包括磷酸化位点的功能和量化方面的信息.预计这个数据库对于预测蛋白磷酸化信息与细胞的生物学表型之间的联系起到重要的作用。

 

3.生物膜的功能蛋白质组学

        生物膜含有多种蛋白,包括各种受体,通道,以及具有催化活性的酶.膜蛋白对于生物的生长,发育,以及对外界环境变化的应答起着极其重要的作用.然而由于膜蛋白的不溶性,对它们的研究远远落后于对可溶性蛋白的研究.我们近期将利用光合蓝藻的thylakoid membrane作为材料,进行膜蛋白的功能蛋白质组学和生物信息学研究. 未来我们还将开展动物细胞生物膜的功能蛋白质组学研究.

发表文章
Ning W, Yu Y, Xu H, Liu X, Wang D, Wang J, Wang Y, Meng W. The CAMSAP3-ACF7 Complex Couples Noncentrosomal Microtubules with Actin Filaments to Coordinate Their Dynamics. Dev Cell. 2016; 39: 61-74.
 
Guan Y, Huang D, Chen F, Gao C, Tao T, Shi H, Zhao S, Liao Z, Lo LJ, Wang Y, Chen J, Peng J. Phosphorylation of Def Regulates Nucleolar p53 Turnover and Cell Cycle Progression through Def Recruitment of Calpain3. PLoS Biol. 2016; 14: e1002555.
 
Li Y, Xu M, Ding X, Yan C, Song Z, Chen L, Huang X, Wang X, Jian Y, Tang G, Tang C, Di Y, Mu S, Liu X, Liu K, Li T, Wang Y, Miao L, Guo W, Hao X, Yang C. Protein kinase C controls lysosome biogenesis independently of mTORC1. Nat Cell Biol. 2016; 18: 1065-77.
 
Jia W, Li B, Li S, Liang Y, Wu X, Ma M, Wang J, Gao J, Cai Y, Zhang Y, Wang Y, Li J, Wang Y. Mitogen-Activated Protein Kinase Cascade MKK7-MPK6 Plays Important Roles in Plant Development and Regulates Shoot Branching by Phosphorylating PIN1 in Arabidopsis. PLoS Biol. 2016; 14: e1002550.
 
Fang L, Ge H, Huang X, Liu Y, Lu M, Wang J, Chen W, Xu W, Wang Y. Trophic Mode-Dependent Proteomic Analysis Reveals Functional Significance of Light-Independent Chlorophyll Synthesis in Synechocystis sp. PCC 6803. Mol Plant. 2016 Aug 29. pii: S1674-2052(16)30167-8.
 
Xie Y, Wang J, Zhang Y, Liu X, Wang X, Liu K, Huang X, Wang Y. Quantitative profiling of spreading-coupled protein tyrosine phosphorylation in migratory cells. Sci. Rep. 2016; 6: 31811.
 
Guan L, Ma X, Zhang J, Liu JJ, Wang Y, Ding M. The Calponin Family Member CHDP-1 Interacts with Rac/CED-10 to Promote Cell Protrusions. PLoS Genet. 2016; 12: e1006163.
 
Wang Y, Liu L, Zhang H, Fan J, Zhang F, Yu M, Shi L, Yang L, Lam SM, Wang H, Chen X, Wang Y, Gao F, Shui G, Xu Z. Mea6 controls VLDL transport through the coordinated regulation of COPII assembly. Cell Res. 2016; 26: 787-804.
 
Derous D, Mitchell SE, Green CL, Wang Y, Han JD, Chen L, Promislow DE, Lusseau1 D, Speakman JR, Douglas A. The effects of graded levels of calorie restriction: VII. Topological rearrangement of hypothalamic aging networks. Aging (Albany NY). 2016; 8: 917-32.
 
Chao Q, Gao ZF, Wang YF, Li Z, Huang XH, Wang YC, Mei YC, Zhao BG, Li L, Jiang YB, Wang BC. The proteome and phosphoproteome of maize pollen uncovers fertility candidate proteins.  Plant Mol Biol. 2016; 91: 287-304.
 
Mitchell SE, Delville C, Konstantopedos P, Derous D, Green CL, Wang Y, Han JJ, Promislow DE, Douglas A, Chen L, Lusseau D, Speakman JR. The effects of graded levels of calorie restriction: V. Impact of short term calorie and protein restriction on physical activity in the C57BL/6 mouse. Oncotarget. 2016; 7: 19147-70.
 
Derous D, Mitchell SE, Green CL, Chen L, Han JJ, Wang Y, Promislow DE, Lusseau D, Speakman JR, Douglas A. The effects of graded levels of calorie restriction: VI. Impact of short-term graded calorie restriction on transcriptomic responses of the hypothalamic hunger and circadian signaling pathways. Aging (Albany NY). 2016; 8: 642-63.
 
Wang Z, Li N, Shan J, Gonzalez N, Huang X, Wang Y, Inze D, and Li Y. SCFSAP controls organ size by targeting PPD proteins for degradation in Arabidopsis thaliana. Nature Communications. 2016; 7:11192.
 
Wang T, Liang L, Xue Y, Jia PF, Chen W, Zhang MX, Wang YC, Li HJ, Yang WC. A receptor heteromer mediates the male perception of female attractants in plants. Nature. 2016; 536: 360.
 
Zhang B, Wang X, Zhao Z, Wang R, Huang X, Zhu Y, Yuan L, Wang Y, Xu X, Burlingame AL, Gao Y, Sun Y, Tang W. OsBRI1 activates BR signaling by preventing binding between the TPR and kinase domains of OsBSK3 via phosphorylation. Plant Physiol. 2016; 170: 1149-61.
 
Wang J, Yu L, Huang X, Wang Y, Zhao J. Comparative proteome analysis of saccular intracranial aneurysms with iTRAQ quantitative proteomics. J Proteomics. 2016; 130:120-8.
 
Lusseau D, Mitchell SE, Barros C, Derous D, Green C, Chen L, Han JD, Wang Y, Promislow DE, Douglas A, Speakman JR. The effects of graded levels of calorie restriction: IV. Non-linear change in behavioural phenotype of mice in response to short-term calorie restriction. Sci Rep. 2015; 5:13198.
 
Mitchell SE, Delville C, Konstantopedos P, Derous D, Green CL, Chen L, Han JD, Wang Y, Promislow DE, Douglas A, Lusseau D, Speakman JR. The effects of graded levels of calorie restriction: III. Impact of short term calorie and protein restriction on mean daily body temperature and torpor use in the C57BL/6 mouse. Oncotarget. 2015; 6:18314-37.
 
Mitchell SE, Tang Z, Kerbois C, Delville C, Konstantopedos P, Bruel A, Derous D, Green C, Aspden RM, Goodyear SR, Chen L, Han JJ, Wang Y, Promislow DE, Lusseau D, Douglas A, Speakman JR. The effects of graded levels of calorie restriction: I. impact of short term calorie and protein restriction on body composition in the C57BL/6 mouse. Oncotarget. 2015;6:15902-30.
 
Mitchell SE, Delville C, Konstantopedos P, Hurst J, Derous D, Green C, Chen L, Han JJ, Wang Y, Promislow DE, Lusseau D, Douglas A, Speakman JR. The effects of graded levels of calorie restriction: II. Impact of short term calorie and protein restriction on circulating hormone levels, glucose homeostasis and oxidative stress in male C57BL/6 mice. Oncotarget. 2015;6:23213-37.
 
Ning DL, Liu KH, Liu CC, Liu JW, Qian CR, Yu Y, Wang YF, Wang YC*, Wang BC. Large-scale comparative phosphoprotein analysis of maize seedling leaves during greening. Planta. 2015 Oct 23. [Epub ahead of print] PMID: 26497871 (*co-corresponding).
 
Bai C, Guo P, Zhao Q, Lv Z, Zhang S, Gao F, Gao L, Wang Y, Tian Z, Wang J, Yang F, Liu C. Protomer roles in Chloroplast Chaperonin Assembly and Function.  Mol Plant. 2015;8(10):1478-92.
 
Gao L, Wang J, Ge H, Fang L, Zhang Y, Huang X, Wang Y. Toward the complete proteome of Synechocystis sp. PCC 6803. Photosynth Res, 2015, 126:203-19.
 
Hu J, Huang X, Chen L, Sun X, Lu C, Zhang L, Wang Y, Zuo J. Site-specific nitrosoproteomic identification of endogenously S-nitrosylated proteins in Arabidopsis. Plant Physiol, 2015;167:1731-46.
 
Gao L, Ge H,  Huang X, Liu K, Zhang Y, Xu W, and Wang Y. Systematically Ranking the Tightness of Membrane Association for Peripheral Membrane Proteins. Mol Cell Proteomics, 2015;14:340-53.
 
Yin P, Tu Z, Yin A, Zhao T, Yan S, Guo X, Chang R, Zhang L, Hong Y, Huang X, Zhou J, Wang Y, Li S, Li XJ. Aged Monkey Brains Reveal the Role of Ubiquitin-Conjugating Enzyme UBE2N in the Synaptosomal Accumulation of Mutant Huntingtin. Hum Mol Genet, 2015; 24:1350-62.
 
Gao L, Huang X, Ge H, Zhang Y, Kang Y, Fang L, Liu K, Wang Y. Profiling and Compositional Analysis of the Exoproteome of Synechocystis sp. PCC 6803. J Metabol Sys Biol, 2014;1(1): 8.
 
Liu K, Zhang J, Fu B, Xie H, Wang Y, Qian X. Evaluation of empirical rule of linearly correlated peptide selection (ERLPS) for proteotypic peptide-based quantitative proteomics. Proteomics, 2014;14:1593-603.
 
Bi J, Wang W, Liu Z, Huang X, Jiang Q, Liu G, Wang Y, Huang X. Seipin promotes adipose tissue fat storage through the ER Ca²⁺-ATPase SERCA. Cell Metab, 2014;19:861-71.
 
Li L, Li M, Yu L, Zhou Z, Liang X, Liu Z, Cai G, Gao L, Zhang X, Wang Y, Chen S, Zhou JM. The FLS2-associated kinase BIK1 directly phosphorylates the NADPH oxidase RbohD to control plant immunity. Cell Host Microbe, 2014;15:329-38.
 
Gao L, Shen C, Liao L, Huang X, Liu K, Wang W, Guo L, Jin W,Huang F, Xu W, and Wang Y. Functional proteomic discovery of Slr0110 as a central regulator of carbohydrate metabolism in Synechocystis sp. PCC6803.  Mol Cell Proteomics, 2014;13:204-19.
 
Chen D, Jian Y, Liu X, Zhang Y, Liang J, Qi X, Du H, Zou W, Chen L, Chai Y, Ou G, Miao L, Wang Y, Yang C. Clathrin and AP2 are required for phagocytic receptor-mediated apoptotic cell clearance in Caenorhabditis  elegans. PLoS Genet, 2013; 9(5):e1003517.
 
Zhai Q, Yan L, Tan D, Chen R, Sun J, Gao L, Dong MQ, Wang Y, Li C. Phosphorylation-coupled proteolysis of the transcription factor MYC2 is important for jasmonate-signaled plant immunity. PLoS Genet, 2013; 9(4):e1003422.
 
Kelber JA, Reno T, Kaushal S, Metildi C, Wright T, Stoletov K, Weems JM, Park FD, Mose E, Wang Y, Hoffman RM, Lowy AM, Bouvet M, Klemke RL, KRas induces a Src/PEAK1/ErbB2 kinase amplification loop that drives metastatic growth and therapy resistance in pancreatic cancer. Cancer Res, 2012, 72: 2554-64.
 
Wang Y, and Klemke R. Proteomics method for identification of pseudopodium phosphotyrosine proteins. Methods Mol Biol, 2012;757:349-65.
 
Xu W, Wang Y*, Taylor E, Laujac A, Gao L, Savikhin S, Chitnis PR. Mutational analysis of photosystem I of Synechocystis sp. PCC 6803: the role of four conserved aromatic residues in the j-helix of PsaB. PLoS One, 2011;6(9):e24625 (*co-corresponding).
 
Wang Y, Yang F, Fu Y, Huang X, Wang W, Jiang X, Gritsenko MA, Zhao R, Monore ME, Pertz OC, Purvine SO, Orton DJ, Jacobs JM, Camp DG 2nd, Smith RD, Klemke RL. Spatial phosphoprotein profiling reveals a compartmentalized extracellular signal-regulated kinase switch governing neurite growth and retraction. J Biol Chem. 2011; 286:18190-201.
 
Wang Y, Yang F, Gritsenko MA, Wang Y, Clauss T, Liu T, Shen Y, Monroe ME, Lopez-Ferrer D, Reno T, Moore RJ, Klemke RL, Camp DG 2nd, Smith RD.  Reversed-phase chromatography with multiple fraction concatenation strategy for proteome profiling of human MCF10A cells.  Proteomics, 2011;11:2019-26.
 
Yang F, Waters KM, Miller JH, Gritsenko MA, Zhao R, Du X, Livesay EA, Purvine SO, Monroe ME, Wang Y, Camp DG 2nd, Smith RD, Stenoien DL. Phosphoproteomics Profiling of Human Skin Fibroblast Cells Reveals Pathways and Proteins Affected by Low Doses of Ionizing Radiation. PLos One, 2010;5: e14152.
 
Wang Y, Kelber JA, Tran Cao HS, Cantin GT, Lin R, Wang W, Kaushal S, Bristow JM, Edgington TS, Hoffman RM, Bouvet M, Yates JR 3rd, Klemke RL. Pseudopodium-enriched atypical kinase 1 regulates the cytoskeleton and cancer progression. Proc Natl Acad Sci U S A, 2010; 107(24):10920-5.
 
Sampathkumar P, Ozyurt SA, Miller SA, Bain KT, Rutter ME, Gheyi T, Abrams B, Wang Y, Atwell S, Luz JG, Thompson DA, Wasserman SR, Emtage JS, Park EC, Rongo C, Jin Y, Klemke RL, Sauder JM, Burley SK. Structures of PHR Domains from Mus musculus Phr1 (Mycbp2) Explain the Loss-of-Function Mutation (Gly1092 → Glu) of the C. elegans Ortholog RPM-1. J Mol Biol, 2010;397(4):883-92.
 
Wang Y*, Xu W*, Chitnis PR. Identification and bioinformatic analysis of the thylakoid membrane proteome of Synechocystis sp. PCC6803.  Proteome Science, 2009, 7: 11. (*corresponding author).
 
Ding SJ*, Wang Y*, Jacobs JM, Qian WJ, Yang F, Tolmachev AV, Du X, Wang W, Moore RJ, Monroe ME, Purvine SO, Waters K, Heibeck TH, Adkins JN, Camp DG 2nd, Klemke RL, Smith RD. Quantitative Phosphoproteome Analysis of Lysophosphatidic Acid induced chemotaxis using Dual-step 18O Labeling Coupled with Immobilized Metal Affinity Chromatography. J Proteome Res, 2008;7:4215-24. (*co-first authors).
 
Pertz OC, Wang Y, Yang F, Wang W, Gay LJ, Gristenko MA, Clauss TR, Anderson DJ, Liu T, Auberry KJ, Camp DG 2nd, Smith RD, Klemke RL. Spatial Mapping of the Neurite and Soma Proteomes Reveals a Functional Cdc42/Rac Regulatory Network. Proc Natl Acad Sci U S A, 2008;105:1931-1936.
 
Wang Y and Klemke RL. PhosphoBlast: A Computational Tool for Comparing Phosphoprotein Signatures among Large Datasets. Mol Cell Proteomics, 2008;7: 145-162.
 
Wang Y, Ding SJ, Wang W, Jacobs JM, Qian WJ, Moore RJ, Yang F, Camp DG 2nd, Smith RD, Klemke RL. Methods for Pseudopodia Purification and Proteomics Analysis. Sci. STKE, 2007; pl4.
 
Wang Y, Ding SJ, Wang W, Jacobs JM, Qian WJ, Moore RJ, Yang F, Camp DG 2nd, Smith RD, Klemke RL. Profiling Signaling Polarity in Chemotactic Cells.  Proc Natl Acad Sci U S A, 2007;104: 8328-8333.
 
Wang Y, and Klemke RL. Biochemical Purification of Pseudopodia from Migratory Cells. Methods Mol Biol, 2007;370:55-66.
 
Wang Y, Hanley R and Klemke RL. Computational methods for comparison of large genomic and proteomic datasets reveals protein markers of metastatic cancer. J Proteome Res, 2006; 5: 907-15.
 
Harvey D, Wang Y, Chitnis PR, Kothari SC. Computational Opportunities in Proteomics Research: Automation in data processing and analysis. High Performance Computing Conference (HPC 2002), December 16-19 in Banglore, India.
 
Xu W, Tang H, Wang Y, Chitnis PR.  Proteins of the cyanobacterial photosystem I. Biochimica  Biophysica Acta, 2001; 1507: 32-40.
 
Hou JM, Boichenko VA, Wang YC, Chitnis PR, Mauzerall D. Thermodynamics of electron transfer in oxygenic photosynthetic reaction center: a photoacoustic study revealed a similarity to bacterial reaction centers on electron transfer in photosystem I in both volume change and entropy. Biochemistry, 2001; 40: 7109-7116.
 
Wang Y, Sun J, Chitnis PR. Proteomic study of the peripheral proteins from thylakoid membranes of the cyanobacterium Synechocystis sp. PCC 6803.  Electrophoresis, 2000;21: 1746-1754.